https://ir.zou.ac.zw/files/original/3c439c804199e308c55541ebc0ceaff1.pdf 93b2272d42ad099f5ad3d6d2a566b74f Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Staff Publications Text A resource consisting primarily of words for reading. Examples include books, letters, dissertations, poems, newspapers, articles, archives of mailing lists. Note that facsimiles or images of texts are still of the genre Text. Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource DETERMINATION AND QUANTIFICATION OF SINIGRIN GLUCOSINOLATES IN ALTERNARIA SOLANI SUSCEPTIBLE TOMATO ( SOLANUM LYCOPERSICUM ) LEAVES TREATED WITH MORINGA ( MORINGA OLEIFERA ) LEAF E… Creator An entity primarily responsible for making the resource CULVER MVUMI ELIZABETH NGADZE DIANA MARAIS ELSA S. DUTOIT JAMESON KUGARA Publisher An entity responsible for making the resource available Archives of Phytopathology and Plant Protection Date A point or period of time associated with an event in the lifecycle of the resource 2018 Description An account of the resource The study investigates the presence and quantity of anti- microbial sinigrin glucosinolates in tomato leaves after spraying them with moringa (Moringa oleifera) leaf extract (MLAE). Moringa concentrates (0.5, 0.75, 1.00 and 1.5 kg L�1 (w v�1 )) were prepared. Distilled water was the control. Sampled tomato leaves were air-dried, freeze-dried and extracted firstly using pure methanol in a hot water bath and then pellet re-extracted using 5 mL of hot aqueous methanol (70% v v�1 ). An ion exchange column, and sul- phatase was used to achieve glucosiodesulphonation. High performance liquid chromatography (HPLC) was employed in the identification and quantitative analysis of the sinigrin glucosinolates. Tomato (Solanum lycopersicum) leaves treated with MLAE revealed highly significant (p < .001) con- tent of sinigrin glucosinolates. The sinigrin standard and the desulphated sinigrin glucosinolates had a 7 s retention time difference; 5 kg L�1 (w v�1 ) resulted in a superior amount of sinigrin in tomato leaves as compared to all the other MLAE concentrations. The study reveals that spraying MLAE on putatively diseased tomato leaves donates specific quantifi- able glucosinolates like sinigrin, which may be involved in defense against tomato diseases and, hence, recommends use of 5 kg L�1 (w v�1 ) for the highest sinigrin defense tag. desulphated sinigrin glucosinolate high performance liquid chromatography Moringa oleifera leaf extract tomato leaves